Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANKRA2 / ANKRA Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110186-100
Catalog Number: LS-G110186
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRA2 (NM_023039), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANKRA2 | ANKRA | RFXANK-like protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_023039
- SwissProt
- Q9H9E1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTSTNLDIG AQLIVEECPS TYSLTGMPDI KIEHPLDPNS EEGSAQGVAM GMKFILPNRF61DMNVCSRFVK SLNEEDSKNI QDQVNSDLEV ASVLFKAECN IHTSPSPGIQ VRHVYTPSTT121KHFSPIKQST TLTNKHRGNE VSTTPLLANS LSVHQLAAQG EMLYLATRIE QENVINHTDE181EGFTPLMWAA AHGQIAVVEF LLQNGADPQL LGKGRESALS LACSKGYTDI VKMLLDCGVD241VNEYDWNGGT PLLYAVHGNH VKCVKMLLES GADPTIETDS GYNSMDLAVA LGYRSVQQVI301ESHLLKLLQN IKETRTRPLEQKLISEEDLAANDILDYKDDDDKV
313 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.