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ANKMY1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKMY1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKMY1 antibodies →
$725.00each
Catalog Number: LS-G112582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKMY1 (NM_016552), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKMY1 | TSAL1 | ZMYND13
Origin Species
Human
NCBI GenBank
NM_016552
SwissProt
Q9P2S6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYQGEFGLNM KLGYGKFSWP TGESYHGQFY RDHCHGLGTY MWPDGSSFTG TFYLSHREGY
61GTMYMKTRLF QGLYKADQRF GPGVETYPDG SQDVGLWFRE QLIKLCTQIP SGFSLLRYPE
121FSSFITHSPA RISLSEEEKT EWGLQEGQDP FFYDYKRFLL NDNLTLPPEM YVYSTNSDHL
181PMTSSFRKEL DARIFLNEIP PFVEDGEPWF IINETPLLVK IQKQTYKFRN KPAHTSWNMG
241AILEGKRSGF APCGPKEQLS MEMILKAEEG NHEWICRILK DNFASADVAD AKGYTVLAAA
301ATHCHNDIVN LLLDCGADVN KCSDEGLTAL SMCFLLHYPA QSFKPNVAER TIPEPQEPPK
361FPVVPILSSS FMDTNLESLY YEVNVPSQGS YELRPPPAPL LLPRVSGSHE GGHFQDTGQC
421GGSIDHRSSS LKGDSPLVKG SLGHVESGLE DVLGNTDRGS LCSAETKFES NVCVCDFSIE
481LSQAMLERSA QSHSLLKMAS PSPCTSSFDK GTMRRMALSM IERRKRWRTI KLLLRRGADP
541NLCCVPMQVL FLAVKAGDVD GVRLLLEHGA RTDICFPPQL STLTPLHIAA ALPGEEGVQI
601VELLLHAITD VDAKASDEDD TYKPGKLDLL PSSLKLSNEP GPPQAYYSTD TALPEEGGRT
661ALHMACERED DNKCARDIVR LLLSHGANPN LLWSGHSPLS LSIASGNELV VKELLTQGAD
721PNLPLTKGLG SALCVACDLT YEHQRNMDSK LALIDRLISH GADILKPVML RQGEKEAVGT
781AVDYGYFRFF QDRRIARCPF HTLMPAERET FLARKRLLEY MGLQLRQAVF AKESQWDPTW
841LYLCKRAELI PSHRMKKKGP SLPRGLDVKE QGQIPFFKFC YQCGRSIGVR LLPCPRCYGI
901LTCSKYCKTK AWTEFHKKDC GDLVAIVTQL EQVSRRREEF Q
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

941 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.