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ANKFY1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKFY1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKFY1 antibodies →
$725.00each
Catalog Number: LS-G112653-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKFY1 (NM_016376), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKFY1 | ANKHZN | ZFYVE14 | BTBD23 | KIAA1255 | Rabankyrin-5
Origin Species
Human
NCBI GenBank
NM_016376
SwissProt
Q9P2R3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEEEVAKLE KHLMLLRQEY VKLQKKLAET EKRCALLAAQ ANKESSSESF ISRLLAIVAD
61LYEQEQYSDL KIKVGDRHIS AHKFVLAARS DSWSLANLSS TKELDLSDAN PEVTMTMLRW
121IYTDELEFRE DDVFLTELMK LANRFQLQLL RERCEKGVMS LVNVRNCIRF YQTAEELNAS
181TLMNYCAEII ASHWDDLRKE DFSSMSAQLL YKMIKSKTEY PLHKAIKVER EDVVFLYLIE
241MDSQLPGKLN EADHNGDLAL DLALSRRLES IATTLVSHKA DVDMVDKSGW SLLHKGIQRG
301DLFAATFLIK NGAFVNAATL GAQETPLHLV ALYSSKKHSA DVMSEMAQIA EALLQAGANP
361NMQDSKGRTP LHVSIMAGNE YVFSQLLQCK QLDLELKDHE GSTALWLAVQ HITVSSDQSV
421NPFEDVPVVN GTSFDENSFA ARLIQRGSHT DAPDTATGNC LLQRAAGAGN EAAALFLATN
481GAHVNHRNKW GETPLHTACR HGLANLTAEL LQQGANPNLQ TEEALPLPKE AASLTSLADS
541VHLQTPLHMA IAYNHPDVVS VILEQKANAL HATNNLQIIP DFSLKDSRDQ TVLGLALWTG
601MHTIAAQLLG SGAAINDTMS DGQTLLHMAI QRQDSKSALF LLEHQADINV SRTQDGETAL
661QLAIRNQLPL VVDAICTRGA DMSVPDEKGN PPLWLALANN LEDIASTLVR HGCDATCWGP
721GPGGCLQTLL HRAIDENNEP TACFLIRSGC DVNSPRQPGA NGEGEEEARD GQTPLHLAAS
781WGLEETVQCL LEFGANVNAQ DAEGRTPIHV AISSQHGVII QLLVSHPDIH LNVRDRQGLT
841PFACAMTFKN NKSAEAILKR ESGAAEQVDN KGRNFLHVAV QNSDIESVLF LISVHANVNS
901RVQDASKLTP LHLAVQAGSE IIVRNLLLAG AKVNELTKHR QTALHLAAQQ DLPTICSVLL
961ENGVDFAAVD ENGNNALHLA VMHGRLNNIR VLLTECTVDA EAFNLRGQSP LHILGQYGKE
1021NAAAIFDLFL ECMPGYPLDK PDADGSTVLL LAYMKGNANL CRAIVRSGAR LGVNNNQGVN
1081IFNYQVATKQ LLFRLLDMLS KEPPWCDGSY CYECTARFGV TTRKHHCRHC GRLLCHKCST
1141KEIPIIKFDL NKPVRVCNIC FDVLTLGGVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1170 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.