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ANKFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKFN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKFN1 antibodies →
$725.00each
Catalog Number: LS-G105884-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKFN1 (NM_153228), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_153228

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEASLTRRLL FKDRHFTCSK IIGRRFACFA QRLSHRRKQS QCDLLNESTG QLPTTCSSAA
61SNSINWNCRV KMTQQMQNLH LCQSKKHSAP SSPNAAKRLY RNLSEKLKGS HSSFDEAYFR
121TRTDRLSLRK TSVNFQGNEA MFEAVEQQDM DAVQILLYQY TPEELDLNTP NSEGLTPLDI
181AIMTNNVPIA RILLRTGARE SPHFVSLESR AMHLNTLVQE AQERVSELSA QVENEGFTLD
241NTEKEKQLKA WEWRYRLYRR MKTGFEHARA PEMPTNVCLM VTSSTSLTVS FQEPLSVNAA
301VVTRYKVEWS MSEDFSPLAG EIIMDNLQTL RCTITGLTMG QQYFVQVSAY NMKGWGPAQT
361TTPACASPSN WKDYDDREPR HKGQSEVLEG LLQQVRALHQ HYSCRESTKL QTTGRKQSVS
421RSLKHLFHSS NKFVKTLKRG LYIAVIFYYK DNILVTNEDQ VPIVEIDDSH TSSITQDFLW
481FTKLSCMWED IRWLRQSIPI SSSSSTVLQT RQKMLAATAQ LQNLLGTHNL GRVYYEPIKD
541RHGNILIVTI REVEMLYSFF NGKWMQISKL QSQRKSLSTP EEPTALDILL ITIQDILSYH
601KRSHQRLFPG LYLGYLKLCS SVDQIKVLVT QKLPNILCHV KIRENNNISR EEWEWIQKLS
661GSESMESVDH TSDCPMQLFF YELQMAVKAL LQQINIPLHQ ARNFRLYTQE VLEMGHNVSF
721LLLLPASDDV CTAPGQNNPY TPHSGFLNLP LQMFELGIVA CFT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

763 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.