LSBio
ANKDD1A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANKDD1A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANKDD1A antibodies →
$725.00each
Catalog Number: LS-G104181-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKDD1A (NM_182703), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANKDD1A
Origin Species
Human
SwissProt
Q495B1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MQEELAWETD GLLPLERQLH EAARQNNVGR MQELIGRRVN TRARNHVGRV ALHWAAGAGH
61EQAVRLLLEH EAAVDEEDAV GALTEARLCF GMNALLLSAW FGHLRILQIL VNSGAKIHCE
121SKDGLTLLHC AAQKGHVPVL AFIMEDLEDV ALDHVDKLGR TAFHRAAEHG QLDALDFLVG
181SGCDHNVKDK EGNTALHLAA GRGHMAVLQR LVDIGLDLEE QNAEGLTALH SAAGGSHPDC
241VQLLLRAGST VNALTQKNLS CLHYAALSGS EDVSRVLIHA GGCANVVDHQ GASPLHLAVR
301HNFPALVRLL INSDSDVNAV DNRQQTPLHL AAEHAWQDIA DMLLIAGVDL NLRDKQGKTA
361LAVAVRSNHV SLVDMIIKAD RFYRWEKDHP SDPSGKSLSF KQDHRQETQQ LRSVLWRLAS
421RYLQPREWKK LAYSWEFTEA HVDAIEQQWT GTRSYQEHGH RMLLIWLHGV ATAGENPSKA
481LFEGLVAIGR RDLAGWSTMA RSQLTATSAS RVQMILVPQP PE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

522 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.