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Anillin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Anillin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANLN antibodies →
$725.00each
Catalog Number: LS-G111665-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Anillin(ANLN) (NM_018685), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Actin-binding protein anillin | ANILLIN | Anillin | actin binding protein | Scraps | Scra | ANLN
Origin Species
Human
NCBI GenBank
NM_018685
SwissProt
Q9NQW6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPFTEKLLE RTRARRENLQ RKMAERPTAA PRSMTHAKRA RQPLSEASNQ QPLSGGEEKS
61CTKPSPSKKR CSDNTEVEVS NLENKQPVES TSAKSCSPSP VSPQVQPQAA DTISDSVAVP
121ASLLGMRRGL NSRLEATAAS SVKTRMQKLA EQRRRWDNDD MTDDIPESSL FSPMPSEEKA
181ASPPKPLLSN ASATPVGRRG RLANLAATIC SWEDDVNHSF AKQNSVQEQP GTACLSKFSS
241ASGASARINS SSVKQEATFC SQRDGDASLN KALSSSADDA SLVNASISSS VKATSSPVKS
301TTSITDAKSC EGQNPELLPK TPISPLKTGV SKPIVKSTLS QTVPSKGELS REICLQSQSK
361DKSTTPGGTG IKPFLERFGE RCQEHSKESP ARSTPHRTPI ITPNTKAIQE RLFKQDTSSS
421TTHLAQQLKQ ERQKELACLR GRFDKGNIWS AEKGGNSKSK QLETKQETHC QSTPLKKHQG
481VSKTQSLPVT EKVTENQIPA KNSSTEPKGF TECEMTKSSP LKITLFLEED KSLKVTSDPK
541VEQKIEVIRE IEMSVDDDDI NSSKVINDLF SDVLEEGELD MEKSQEEMDQ ALAESSEEQE
601DALNISSMSL LAPLAQTVGV VSPESLVSTP RLELKDTSRS DESPKPGKFQ RTRVPRAESG
661DSLGSEDRDL LYSIDAYRSQ RFKETERPSI KQVIVRKEDV TSKLDEKNNA FPCQVNIKQK
721MQELNNEINM QQTVIYQASQ ALNCCVDEEH GKGSLEEAEA ERLLLIATGK RTLLIDELNK
781LKNEGPQRKN KASPQSEFMP SKGSVTLSEI RLPLKADFVC STVQKPDAAN YYYLIILKAG
841AENMVATPLA STSNSLNGDA LTFTTTFTLQ DVSNDFEINI EVYSLVQKKD PSGLDKKKKT
901SKSKAITPKR LLTSITTKSN IHSSVMASPG GLSAVRTSNF ALVGSYTLSL SSVGNTKFVL
961DKVPFLSSLE GHIYLKIKCQ VNSSVEERGF LTIFEDVSGF GAWHRRWCVL SGNCISYWTY
1021PDDEKRKNPI GRINLANCTS RQIEPANREF CARRNTFELI TVRPQREDDR ETLVSQCRDT
1081LCVTKNWLSA DTKEERDLWM QKLNQVLVDI RLWQPDACYK PIGKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1125 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.