Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ANGPTL7 / CDT6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101730-100
Catalog Number: LS-G101730
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Angiopoietin like 7(ANGPTL7) (NM_021146), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Angiopoietin-like 7 | Angiopoietin-like protein 7 | Angiopoietin-like factor | ANGPTL7 | Angiopoietin-related protein 7 | CDT6 | DJ647M16.1 | AngX | RP4-647M16.2
- Origin Species
- Human
- NCBI GenBank
- NM_021146
- SwissProt
- O43827
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLKKPLSAVT WLCIFIVAFV SHPAWLQKLS KHKTPAQPQL KAANCCEEVK ELKAQVANLS61SLLSELNKKQ ERDWVSVVMQ VMELESNSKR MESRLTDAES KYSEMNNQID IMQLQAAQTV121TQTSADAIYD CSSLYQKNYR ISGVYKLPPD DFLGSPELEV FCDMETSGGG WTIIQRRKSG181LVSFYRDWKQ YKQGFGSIRG DFWLGNEHIH RLSRQPTRLR VEMEDWEGNL RYAEYSHFVL241GNELNSYRLF LGNYTGNVGN DALQYHNNTA FSTKDKDNDN CLDKCAQLRK GGYWYNCCTD301SNLNGVYYRL GEHNKHLDGI TWYGWHGSTY SLKRVEMKIR PEDFKPTRTR PLEQKLISEE361DLAANDILDY KDDDDKV
377 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.