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ANGEL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANGEL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANGEL2 antibodies →
$725.00each
Catalog Number: LS-G106987-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANGEL2 (NM_144567), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANGEL2 | KIAA0759L | Protein angel homolog 2 | Angel homolog 2 (Drosophila)
Origin Species
Human
NCBI GenBank
AL079275
SwissProt
Q5VTE6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEAWRCVRKG YGHCVVGRGR YPMFPHHSRS LGRDWTTPWE NLQRCCWNRH ISSCMRWPGH
61YSRAPYPYFS SRHFSLNWRP PCLFESRTQF QYCNWRPDNL SQTSLIHLSS YVMNAEGDEP
121SSKRRKHQGV IKRNWEYICS HDKEKTKILG DKNVDPKCED SENKFDFSVM SYNILSQDLL
181EDNSHLYRHC RRPVLHWSFR FPNILKEIKH FDADVLCLQE VQEDHYGAEI RPSLESLGYH
241CEYKMRTGRK PDGCAICFKH SKFSLLSVNP VEFFRPDISL LDRDNVGLVL LLQPKIPYAA
301CPAICVANTH LLYNPRRGDI KLTQLAMLLA EISSVAHQKD GSFCPIVMCG DFNSVPGSPL
361YSFIKEGKLN YEGLPIGKVS GQEQSSRGQR ILSIPIWPPN LGISQNCVYE VQQVPKVEKT
421DSDLTQTQLK QTEVLVTAEK LSSNLQHHFS LSSVYSHYFP DTGIPEVTTC HSRSAITVDY
481IFYSAEKEDV AGHPGAEVAL VGGLKLLARL SLLTEQDLWT VNGLPNENNS SDHLPLLAKF
541RLEL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

544 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.