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ANGEL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ANGEL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ANGEL1 antibodies →
$504.00each
Catalog Number: LS-G113309-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANGEL1 (NM_015305), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANGEL1 | KIAA0759 | Angel homolog 1 (Drosophila) | Protein angel homolog 1
Origin Species
Human
SwissProt
Q9UNK9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIASCLCYLL LPATRLFRAL SDAFFTCRKN VLLANSSSPQ VEGDFAMAPR GPEQEECEGL
61LQQWREEGLS QVLSTASEGP LIDKGLAQSS LALLMDNPGE ENAASEDRWS SRQLSDLRAA
121ENLDEPFPEM LGEEPLLEVE GVEGSMWAAI PMQSEPQYAD CAALPVGALA TEQWEEDPAV
181LAWSIAPEPV PQEEASIWPF EGLGQLQPPA VEIPYHEILW REWEDFSTQP DAQGLKAGDG
241PQFQFTLMSY NILAQDLMQQ SSELYLHCHP DILNWNYRFV NLMQEFQHWD PDILCLQEVQ
301EDHYWEQLEP SLRMMGFTCF YKRRTGCKTD GCAVCYKPTR FRLLCASPVE YFRPGLELLN
361RDNVGLVLLL QPLVPEGLGQ VSVAPLCVAN THILYNPRRG DVKLAQMAIL LAEVGKVARL
421SDGSHCPIIL CGDLNSVPDS PLYNFIRDGE LQYHGMPAWK VSGQEDFSHQ LYQRKLQAPL
481WPSSLGITDC CQYVTSCHPK RSERRKYGRD FLLRFRFCSI ACQRPVGLVL MEGVTDTKPE
541RPAGWAESVL EEDASELEPA FSRTVGTIQH CLHLTSVYTH FLPQRGRPEV TTMPLGLGMT
601VDYIFFSAES CENGNRTDHR LYRDGTLKLL GRLSLLSEEI LWAANGLPNP FCSSDHLCLL
661ASFGMEVTAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

670 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.