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AMPD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AMPD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AMPD2 antibodies →
$504.00each
Catalog Number: LS-G107098-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMPD2 (NM_139156), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AMP deaminase isoform L | AMP deaminase 2 | AMPD | AMPD2
Origin Species
Human
NCBI GenBank
NM_004037
SwissProt
Q01433

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
91.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASEARGGLG APPLQSARSL PGPAPCLKHF PLDLRTSMDG KCKEIAEELF TRSLAESELR
61SAPYEFPEES PIEQLEERRQ RLERQISQDV KLEPDILLRA KQDFLKTDSD SDLQLYKEQG
121EGQGDRSLRE RDVLEREFQR VTISGEEKCG VPFTDLLDAA KSVVRALFIR EKYMALSLQS
181FCPTTRRYLQ QLAEKPLETR TYEQGPDTPV SADAPVHPPA LEQHPYEHCE PSTMPGDLGL
241GLRMVRGVVH VYTRREPDEH CSEVELPYPD LQEFVADVNV LMALIINGPI KSFCYRRLQY
301LSSKFQMHVL LNEMKELAAQ KKVPHRDFYN IRKVDTHIHA SSCMNQKHLL RFIKRAMKRH
361LEEIVHVEQG REQTLREVFE SMNLTAYDLS VDTLDVHADR NTFHRFDKFN AKYNPIGESV
421LREIFIKTDN RVSGKYFAHI IKEVMSDLEE SKYQNAELRL SIYGRSRDEW DKLARWAVMH
481RVHSPNVRWL VQVPRLFDVY RTKGQLANFQ EMLENIFLPL FEATVHPASH PELHLFLEHV
541DGFDSVDDES KPENHVFNLE SPLPEAWVEE DNPPYAYYLY YTFANMAMLN HLRRQRGFHT
601FVLRPHCGEA GPIHHLVSAF MLAENISHGL LLRKAPVLQY LYYLAQIGIA MSPLSNNSLF
661LSYHRNPLPE YLSRGLMVSL STDDPLQFHF TKEPLMEEYS IATQVWKLSS CDMCELARNS
721VLMSGFSHKV KSHWLGPNYT KEGPEGNDIR RTNVPDIRVG YRYETLCQEL ALITQAVQSE
781MLETIPEEAG ITMSPGPQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

798 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.