LSBio
AMOTL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AMOTL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AMOTL2 antibodies →
$725.00each
Catalog Number: LS-G112806-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMOTL2 (NM_016201), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Angiomotin-like protein 2 | AMOTL2 | Angiomotin like 2 | LCCP | Leman coiled-coil protein | KIAA0989
Origin Species
Human
NCBI GenBank
NM_016201
SwissProt
Q9Y2J4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
85.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRTLEDSSGT VLHRLIQEQL RYGNLTETRT LLAIQQQALR GGAGTGGTGS PQASLEILAP
61EDSQVLQQAT RQEPQGQEHQ GGENHLAENT LYRLCPQPSK GEELPTYEEA KAHSQYYAAQ
121QAGTRPHAGD RDPRGAPGGS RRQDEALREL RHGHVRSLSE RLLQLSLERN GARAPSHMSS
181SHSFPQLARN QQGPPLRGPP AEGPESRGPP PQYPHVVLAH ETTTAVTDPR YRARGSPHFQ
241HAEVRILQAQ VPPVFLQQQQ QYQYLQQSQE HPPPPHPAAL GHGPLSSLSP PAVEGPVSAQ
301ASSATSGSAH LAQMEAVLRE NARLQRDNER LQRELESSAE KAGRIEKLES EIQRLSEAHE
361SLTRASSKRE ALEKTMRNKM DSEMRRLQDF NRDLRERLES ANRRLASKTQ EAQAGSQDMV
421AKLLAQSYEQ QQEQEKLERE MALLRGAIED QRRRAELLEQ ALGNAQGRAA RAEEELRKKQ
481AYVEKVERLQ QALGQLQAAC EKREQLELRL RTRLEQELKA LRAQQRQAGA PGGSSGSGGS
541PELSALRLSE QLREKEEQIL ALEADMTKWE QKYLEERAMR QFAMDAAATA AAQRDTTLIR
601HSPQPSPSSS FNEGLLTGGH RHQEMESRLK VLHAQILEKD AVIKVLQQRS RRDPGKAIQG
661SLRPAKSVPS VFAAAAAGTQ GWQGLSSSER QTADAPARLT TADRAPTEEP VVTAPPAAHA
721KHGSRDGSTQ TEGPPDSTST CLPPEPDSLL GCSSSQRAAS LDSVATSRVQ DLSDMVEILI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

780 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.