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AMIGO3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AMIGO3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103557-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMIGO3 (NM_198722), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALI3 | AMIGO-3 | Amphoterin-induced protein 3 | Alivin-3 | AMIGO3 | KIAA1851
Origin Species
Human
SwissProt
Q86WK7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTWLVLLGTL LCMLRVGLGT PDSEGFPPRA LHNCPYKCIC AADLLSCTGL GLQDVPAELP
61AATADLDLSH NALQRLRPGW LAPLFQLRAL HLDHNELDAL GRGVFVNASG LRLLDLSSNT
121LRALGRHDLD GLGALEKLLL FNNRLVHLDE HAFHGLRALS HLYLGCNELA SFSFDHLHGL
181SATHLLTLDL SSNRLGHISV PELAALPAFL KNGLYLHNNP LPCDCRLYHL LQRWHQRGLS
241AVRDFAREYV CLAFKVPASR VRFFQHSRVF ENCSSAPALG LERPEEHLYA LVGRSLRLYC
301NTSVPAMRIA WVSPQQELLR APGSRDGSIA VLADGSLAIG NVQEQHAGLF VCLATGPRLH
361HNQTHEYNVS VHFPRPEPEA FNTGFTTLLG CAVGLVLVLL YLFAPPCRCC RRACRCRRWP
421QTPSPLQELS AQSSVLSTTP PDAPSRKASV HKHVVFLEPG RRGLNGRVQL AVAEEFDLYN
481PGGLQLKAGS ESASSIGSEG PMTT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

504 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.