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AMIGO Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AMIGO Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AMIGO1 antibodies →
$504.00each
Catalog Number: LS-G101694-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMIGO1 (NM_020703), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALI2 | AMIGO1 | AMIGO-1 | Amphoterin-induced protein 1 | KIAA1163 | Alivin-2 | AMIGO
Origin Species
Human
NCBI GenBank
AB032989
SwissProt
Q86WK6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHPHRDPRGL WLLLPSLSLL LFEVARAGRA VVSCPAACLC ASNILSCSKQ QLPNVPHSLP
61SYTALLDLSH NNLSRLRAEW TPTRLTQLHS LLLSHNHLNF ISSEAFSPVP NLRYLDLSSN
121QLRTLDEFLF SDLQVLEVLL LYNNHIMAVD RCAFDDMAQL QKLYLSQNQI SRFPLELVKE
181GAKLPKLTLL DLSSNKLKNL PLPDLQKLPA WIKNGLYLHN NPLNCDCELY QLFSHWQYRQ
241LSSVMDFQED LYCMNSKKLH NVFNLSFLNC GEYKERAWEA HLGDTLIIKC DTKQQGMTKV
301WVTPSNERVL DEVTNGTVSV SKDGSLLFQQ VQVEDGGVYT CYAMGETFNE TLSVELKVHN
361FTLHGHHDTL NTAYTTLVGC ILSVVLVLIY LYLTPCRCWC RGVEKPSSHQ GDSLSSSMLS
421TTPNHDPMAG GDKDDGFDRR VAFLEPAGPG QGQSGKLKPG NTLPVPEATG KGQRRMSDPE
481SVSSVFSDTP IVV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.