Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AMID / AIFM2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102096-100
Catalog Number: LS-G102096
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMID(AIFM2) (NM_032797), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIFM2 | Apoptosis-inducing factor 2 | AMID | p53-responsive gene 3 protein | RP11-367H5.2
- Origin Species
- Human
- NCBI GenBank
- NM_032797
- SwissProt
- Q9BRQ8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGSQVSVESG ALHVVIVGGG FGGIAAASQL QALNVPFMLV DMKDSFHHNV AALRASVETG61FAKKTFISYS VTFKDNFRQG LVVGIDLKNQ MVLLQGGEAL PFSHLILATG STGPFPGKFN121EVSSQQAAIQ AYEDMVRQVQ RSRFIVVVGG GSAGVEMAAE IKTEYPEKEV TLIHSQVALA181DKELLPSVRQ EVKEILLRKG VQLLLSERVS NLEELPLNEY REYIKVQTDK GTEVATNLVI241LCTGIKINSS AYRKAFESRL ASSGALRVNE HLQVEGHSNV YAIGDCADVR TPKMAYLAGL301HANIAVANIV NSVKQRPLQA YKPGALTFLL SMGRNDGVGQ ISGFYVGRLM VRLTKSRDLF361VSTSWKTMRQ SPPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
373 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.