Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AMER3 / FAM123C Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106054-100
Catalog Number: LS-G106054
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM123C(AMER3) (NM_152698), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_152698
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 90.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MELKRGKTFI KSSLQVSHEK PPDPAAVAAA REGTGPWSVL PGGQQRPHSE KGPQASPSAQ61EYDRCPNKGA QLDPKGGPAA LCGATFKPVR KCKTHDSMSG AGRATAATGQ LVGSASFPGS121PGSRRMIDYR HFVPQMPFVP AVAKSIPRKR ISLKRPKKCF RNLFHIRRNK TEDLASLAAE181GKSLPSPGDP SDPGGRRSKA FLPPGEGPGL DGLCQDLLDS ELLADASFGL CRALCEDVAS241LQSFDSLTGC GEVFADESSV PSLELNEGPE SPTQAAQGLE SKVPRGPLQG SVEQLASPAQ301NEASDFTRFW DSVNRSVRQQ QRALLGPWLS GPQGTDRDQS RLDTAGLAEL PLCPCRDPRS361GSKASSIDTG TPKSEQPESV STSDEGYYDS FSPGLEEDKK EAESPGTPAA TFPRDSYSGD421ALYELFHDPS EGPLGPSPDD DLCVSESLSG PALGTPLSIC SFRVGAEENL APAPGPDLLS481QGFLQSSWKG KECLLKLCDT ELAITMGIVS WLRRGPTPRA PPTPGQPAAP PGSQGAPRAP541TEKLGGREGL ASDAGGATVC SAPSRQELWA HPGTTGLLAG ESKALGGATQ GTGTLSRDAS601REEETRGHSE GLFSSMESAA TSTTDTSGKN KAPVPSTWPC SQKEPGPPGV LGCFRGPWRP661GHGGDTLDAE PMLAGCVARV AALKISSNEQ PPAAWPPRQD MGSGLFGQRW ARGPDMLEQK721QSSSSPSMTT IHGLPYSAST QDQRCRDRVQ DLSWLRVEPT GLGVQAWASV EDQPLQLSTE781AVEQVAHGSQ LDSEPRSAPA ARWSSQGHHP ESLGLTLNSQ QEGGVSASAP ECRCSLLARE841GLLCGQPEVG ASGPAMAEPH LSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
861 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.