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AMBRA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AMBRA1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AMBRA1 antibodies →
$504.00each
Catalog Number: LS-G112249-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AMBRA1 (NM_017749), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Autophagy/beclin-1 regulator 1 | KIAA1736 | AMBRA1 | WD repeat domain 94 | WDR94 | DCAF3
Origin Species
Human
NCBI GenBank
AK023197
SwissProt
Q9C0C7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKVVPEKNAV RILWGRERGA RAMGAQRLLQ ELVEDKTRWM KWEGKRVELP DSPRSTFLLA
61FSPDRTLLAS THVNHNIYIT EVKTGKCVHS LIGHRRTPWC VTFHPTISGL IASGCLDGEV
121RIWDLHGGSE SWFTDSNNAI ASLAFHPTAQ LLLIATANEI HFWDWSRREP FAVVKTASEM
181ERVRLVRFDP LGHYLLTAIV NPSNQQGDDE PEIPIDGTEL SHYRQRALLQ SQPVRRTPLL
241HNFLHMLSSR SSGIQTEPFH PPEQASSTQQ DQGLLNRPSA FSTVQSSTAG NTLRNLSLGP
301TRRSLGGPLS SHPSRYHREI APGLTGSEWT RTVLSLNSRS EAESMPPPRT SASSVSLLSV
361LRQQEGGSQA SVYTSATEGR GFPASGLATE SDGGNGSSQN NSGSIRHELQ CDLRRFFLEY
421DRLQELDQSL SGEAPQTQQA QEMLNNNIES ERPGPSHQPT PHSSENNSNL SRGHLNRCRA
481CHNLLTFNND TLRWERTTPN YSSGEASSSW QVPSSFESVP SSGSQLPPLE RTEGQTPSSS
541RLELSSSASP QEERTVGVAF NQETGHWERI YTQSSRSGTV SQEALHQDMP EESSEEDSLR
601RRLLESSLIS LSRYDGAGSR EHPIYPDPAR LSPAAYYAQR MIQYLSRRDS IRQRSMRYQQ
661NRLRSSTSSS SSDNQGPSVE GTDLEFEDFE DNGDRSRHRA PRNARMSAPS LGRFVPRRFL
721LPEYLPYAGI FHERGQPGLA THSSVNRVLA GAVIGDGQSA VASNIANTTY RLQWWDFTKF
781DLPEISNASV NVLVQNCKIY NDASCDISAD GQLLAAFIPS SQRGFPDEGI LAVYSLAPHN
841LGEMLYTKRF GPNAISVSLS PMGRYVMVGL ASRRILLHPS TEHMVAQVFR LQQAHGGETS
901MRRVFNVLYP MPADQRRHVS INSARWLPEP GLGLAYGTNK GDLVICRPEA LNSGVEYYWD
961QLNETVFTVH SNSRSSERPG TSRATWRTDR DMGLMNAIGL QPRNPATSVT SQGTETLALQ
1021LQNAETQTER EVPEPGTAAS GPGEGEGSEY GASGEDALSR IQRLMAEGGM TAVVQREQST
1081TMASMGGFGN NIIVSHRIHR SSQTGTEPGA AHTSSPQPST SRGLLPEAGQ LAERGLSPRT
1141ASWDQPGTPG REPTQPTLPS SSPVPIPVSL PSAEGPTVHC ELTNNNHLLD GGSSRGDAAG
1201PRGEPRNR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1208 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.