Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ALX / HSH2D Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108584-100
Catalog Number: LS-G108584
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HSH2D (NM_032855), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ALX | Hematopoietic SH2 protein | HSH2 | HSH2D
- Origin Species
- Human
- NCBI GenBank
- NM_032855
- SwissProt
- Q96JZ2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 38.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTEAGKLPLP LPPRLDWFVH TQMGQLAQDG VPEWFHGAIS REDAENLLES QPLGSFLIRV61SHSHVGYTLS YKAQSSCCHF MVKLLDDGTF MIPGEKVAHT SLDALVTFHQ QKPIEPRREL121LTQPCRQKDP ANVDYEDLFL YSNAVAEEAA CPVSAPEEAS PKPVLCHQSK ERKPSAEMNR181ITTKEATSSC PPKSPLGETR QKLWRSLKML PERGQRVRQQ LKSHLATVNL SSLLDVRRST241VISGPGTGKG SQDHSGDPTS GDRGYTDPCV ATSLKSPSQP QAPKDRKVPT RKAERSVSCI301EVTPGDRSWH QMVVRALSSQ ESKPEHQGLA EPENDQLPEE YQQPPPFAPG YCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
352 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.