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ALPK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ALPK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ALPK3 antibodies →
$725.00each
Catalog Number: LS-G111017-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALPK3 (NM_020778), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Alpha-kinase 3 | KIAA1330 | MIDORI | Alpha-protein kinase 3 | ALPK3 | Muscle alpha-protein kinase | Muscle alpha-kinase
Origin Species
Human
NCBI GenBank
AY044449
SwissProt
Q96L96

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
201.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVAWLVYVL GQQPLARQGE GQSRLVPGRG LVLWLPGLPR SSPSWPAVDL APLAPARPRG
61PLICHTGHEQ AGREPGPGSS TKGPVLHDQD TRCAFLPRPP GPLQTRRYCR HQGRQGSGLG
121AGPGAGTWAP APPGVSKPRC PGRARPGEGQ QQVTTARPPA INRGARQPRA GAAAAGRGPG
181AGAWRTGEAA ASAGPAVGEG GAMGSRRAPT RGWGAGGRSG AGGDGEDDGP VWIPSPASRS
241YLLSVRPETS LSSNRLSHPS SGRSTFCSII AQLTEETQPL FETTLKSRSV SEDSDVRFTC
301IVTGYPEPEV TWYKDDTELD RYCGLPKYEI THQGNRHTLQ LYRCREEDAA IYQASAQNSK
361GIVSCSGVLE VGTMTEYKIH QRWFAKLKRK AAAKLREIEQ SWKHEKAVPG EVDTLRKLSP
421DRFQRKRRLS GAQAPGPSVP TREPEGGTLA AWQEGETETA QHSGLGLINS FASGEVTTNG
481EAAPENGEDG EHGLLTYICD AMELGPQRAL KEESGAKKKK KDEESKQGLR KPELEKAAQS
541RRSSENCIPS SDEPDSCGTQ GPVGVEQVQT QPRGRAARGP GSSGTDSTRK PASAVGTPDK
601AQKAPGPGPG QEVYFSLKDM YLENTQAVRP LGEEGPQTLS VRAPGESPKG KAPLRARSEG
661VPGAPGQPTH SLTPQPTRPF NRKRFAPPKP KGEATTDSKP ISSLSQAPEC GAQSLGKAPP
721QASVQVPTPP ARRRHGTRDS TLQGQAGHRT PGEVLECQTT TAPTMSASSS SDVASIGVST
781SGSQGIIEPM DMETQEDGRT SANQRTGSKK NVQADGKIQV DGRTRGDGTQ TAQRTRADRK
841TQVDAGTQES KRPQSDRSAQ KGMMTQGRAE TQLETTQAGE KIQEDRKAQA DKGTQEDRRM
901QGEKGMQGEK GTQSEGSAPT AMEGQSEQEV ATSLGPPSRT PKLPPTAGPR APLNIECFVQ
961TPEGSCFPKK PGCLPRSEEA VVTASRNHEQ TVLGPLSGNL MLPAQPPHEG SVEQVGGERC
1021RGPQSSGPVE AKQEDSPFQC PKEERPGGVP CMDQGGCPLA GLSQEVPTMP SLPGTGLTAS
1081PKAGPCSTPT SQHGSTATFL PSEDQVLMSS APTLHLGLGT PTQSHPPETM ATSSEGACAQ
1141VPDVEGRTPG PRSCDPGLID SLKNYLLLLL KLSSTETSGA GGESQVGAAT GGLVPSATLT
1201PTVEVAGLSP RTSRRILERV ENNHLVQSAQ TLLLSPCTSR RLTGLLDREV QAGRQALAAA
1261RGSWGPGPSS LTVPAIVVDE EDPGLASEGA SEGEGEVSPE GPGLLGASQE SSMAGRLGEA
1321GGQAAPGQGP SAESIAQEPS QEEKFPGEAL TGLPAATPEE LALGARRKRF LPKVRAAGDG
1381EATTPEERES PTVSPRGPRK SLVPGSPGTP GRERRSPTQG RKASMLEVPR AEEELAAGDL
1441GPSPKAGGLD TEVALDEGKQ ETLAKPRKAK DLLKAPQVIR KIRVEQFPDA SGSLKLWCQF
1501FNILSDSVLT WAKDQRPVGE VGRSAGDEGP AALAIVQASP VDCGVYRCTI HNEHGSASTD
1561FCLSPEVLSG FISREEGEVG EEIEMTPMVF AKGLADSGCW GDKLFGRLVS EELRGGGYGC
1621GLRKASQAKV IYGLEPIFES GRTCIIKVSS LLVFGPSSET SLVGRNYDVT IQGCKIQNMS
1681REYCKIFAAE ARAAPGFGEV PEIIPLYLIY RPANNIPYAT LEEDLGKPLE SYCSREWGCA
1741EAPTASGSSE AMQKCQTFQH WLYQWTNGSF LVTDLAGVDW KMTDVQIATK LRGYQGLKES
1801CFPALLDRFA SSHQCNAYCE LLGLTPLKGP EAAHPQAKAK GSKSPSAGRK GSQLSPQPQK
1861KGLPSPQGTR KSAPSSKATP QASEPVTTQL LGQPPTQEEG SKAQGMR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1907 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.