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ALG9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ALG9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ALG9 antibodies →
$504.00each
Catalog Number: LS-G109776-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALG9 (NM_024740), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALG9 | CDG1L | DIBD1 | LOH11CR1J
Origin Species
Human
NCBI GenBank
NM_024740
SwissProt
Q9H6U8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASRGARQRL KGSGASSGDT APAADKLREL LGSREAGGAE HRTELSGNKA GQVWAPEGST
61AFKCLLSARL CAALLSNISD CDETFNYWEP THYLIYGEGF QTWEYSPAYA IRSYAYLLLH
121AWPAAFHARI LQTNKILVFY FLRCLLAFVS CICELYFYKA VCKKFGLHVS RMMLAFLVLS
181TGMFCSSSAF LPSSFCMYTT LIAMTGWYMD KTSIAVLGVA AGAILGWPFS AALGLPIAFD
241LLVMKHRWKS FFHWSLMALI LFLVPVVVID SYYYGKLVIA PLNIVLYNVF TPHGPDLYGT
301EPWYFYLING FLNFNVAFAL ALLVLPLTSL MEYLLQRFHV QNLGHPYWLT LAPMYIWFII
361FFIQPHKEER FLFPVYPLIC LCGAVALSAL QHSFLYFQKC YHFVFQRYRL EHYTVTSNWL
421ALGTVFLFGL LSFSRSVALF RGYHGPLDLY PEFYRIATDP TIHTVPEGRP VNVCVGKEWY
481RFPSSFLLPD NWQLQFIPSE FRGQLPKPFA EGPLATRIVP TDMNDQNLEE PSRYIDISKC
541HYLVDLDTMR ETPREPKYSS NKEEWISLAY RPFLDASRSS KLLRAFYVPF LSDQYTVYVN
601YTILKPRKAK QIRKKSGG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

618 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.