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ALAS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ALAS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120550-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALAS2 (NM_001037968), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALASE | ANH1 | ASB | Delta-ALA synthetase | ALAS-E | ALAS2 | XLDPP | XLSA | Delta-ALA synthase 2 | XLEPP
Origin Species
Human
NCBI GenBank
NM_000032
SwissProt
P22557

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.49 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGGEVALGW NKKKRLFCED FRFKMVTAAM LLQCCPVLAR GPTSLLGKVV KTHQFLFGIG
61RCPILATQGP NCSQIHLKAT KAGGDSPSWA KGHCPFMLSE LQDGKSKIVQ KAAPEVQEDV
121KAFKTGNYVF SYDQFFRDKI MEKKQDHTYR VFKTVNRWAD AYPFAQHFSE ASVASKDVSV
181WCSNDYLGMS RHPQVLQATQ ETLQRHGAGA GGTRNISGTS KFHVELEQEL AELHQKDSAL
241LFSSCFVAND STLFTLAKIL PGCEIYSDAG NHASMIQGIR NSGAAKFVFR HNDPDHLKKL
301LEKSNPKIPK IVAFETVHSM DGAICPLEEL CDVSHQYGAL TFVDEVHAVG LYGSRGAGIG
361ERDGIMHKID IISGTLGKAF GCVGGYIAST RDLVDMVRSY AAGFIFTTSL PPMVLSGALE
421SVRLLKGEEG QALRRAHQRN VKHMRQLLMD RGLPVIPCPS HIIPIRVGNA ALNSKLCDLL
481LSKHGIYVQA INYPTVPRGE ELLRLAPSPH HSPQMMEDFV EKLLLAWTAV GLPLQDVSVA
541ACNFCRRPVH FELMSEWERS YFGNMGPQYV TTYA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

574 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.