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ALAD Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ALAD Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ALAD antibodies →
$504.00each
Catalog Number: LS-G123434-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ALAD (NM_000031), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ALAD | ALADH | Aminolevulinate dehydratase | PBGS | Porphobilinogen synthase
Origin Species
Hu
NCBI GenBank
NM_000031
SwissProt
P13716

Protein

Expression System
HEK293
Fusion Tag
FLAG, Myc
Molecular Weight
37.23 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPLCPLAHAM QPQSVLHSGY FHPLLRAWQT ATTTLNASNL IYPIFVTDVP DDIQPITSLP
61GVARYGVKRL EEMLRPLVEE GLRCVLIFGV PSRVPKDERG SAADSEESPA IEAIHLLRKT
121FPNLLVACDV CLCPYTSHGH CGLLSENGAF RAEESRQRLA EVALAYAKAG CQVVAPSDMM
181DGRVEAIKEA LMAHGLGNRV SVMSYSAKFA SCFYGPFRDA AKSSPAFGDR RCYQLPPGAR
241GLALRAVDRD VREGADMLMV KPGMPYLDIV REVKDKHPDL PLAVYHVSGE FAMLWHGAQA
301GAFDLKAAVL EAMTAFRRAG ADIIITYYTP QLLQWLKEES GPTRTRRLEQ KLISEEDLAA
361NDILDYKDDD DKV

373 aa

Format

Purity
Unpurified
Conjugate/Label
Unconj
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.