Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AKTIP / FTS Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121379-100
Catalog Number: LS-G121379
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FTS(AKTIP) (NM_001012398), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AKT interacting protein | AKT-interacting protein | AKTIP | FT1 | Fused toes (mouse) homolog | Fused toes homolog | Fused toes homolog (mouse) | FTS | Fused toes protein homolog
- Origin Species
- Human
- NCBI GenBank
- NM_001012398
- SwissProt
- Q9H8T0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 32.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPFWSMSTS SVRKRSEGEE KTLTGDVKTS PPRTAPKKQL PSIPKNALPI TKPTSPAPAA61QSTNGTHASY GPFYLEYSLL AEFTLVVKQK LPGVYVQPSY RSALMWFGVI FIRHGLYQDG121VFKFTVYIPD NYPDGDCPRL VFDIPVFHPL VDPTSGELDV KRAFAKWRRN HNHIWQVLMY181ARRVFYKIDT ASPLNPEAAV LYEKDIQLFK SNVVDSVKVC TARLFDQPKI EDPYAISFSP241WNPSVHDEAR EKMLTQKKKP EEQHNKSVHV AGLSWVKPGS VQPFSKEEKT VATTRTRPLEQKLISEEDLAANDILDYKDDDDKV
293 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.