Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AKT1S1 / PRAS40 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108862-100
Catalog Number: LS-G108862
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PRAS40(AKT1S1) (NM_032375), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AKT1S1 | PRAS40 | Proline-rich AKT1 substrate 1 | Lobe
- Origin Species
- Human
- NCBI GenBank
- NM_032375
- SwissProt
- Q96B36
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 27.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MASGRPEELW EAVVGAAERF RARTGTELVL LTAAPPPPPR PGPCAYPAHG RGALAEAARR61CLHDIALAHR AATAARPPAP PPAPQPPSPT PSPPRPTLAR EDNEEDEDEP TETETSGEQL121GISDNGGLFV MDEDATLQDL PPFCESDPES TDDGSLSEET PAGPPTCSVP PASALPTQQY181AKSLPVSVPV WGFKEKRTEA RSSDEENGPP SSPDLDRIAA SMRALVLREA EDTQVFGDLP241RPRLNTSDFQ KLKRKYTRTR PLEQKLISEE DLAANDILDY KDDDDKV
287 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.