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AKNAD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AKNAD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106040-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AKNAD1 (NM_152763), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AKNA domain containing 1 | AKNAD1 | C1orf62 | Protein AKNAD1 | RP11-475E11.3
Origin Species
Human
NCBI GenBank
NM_152763
SwissProt
Q5T1N1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDEADFSEHT TYKQEDLPYD GDLSQIKIGN DYSFTSKKDG LEVLNQIIFI ADDPQEKAMH
61SETCGNTAVT IPLGKITENA ANKKDEKEKQ CTAALHIPAN EGDASKSSIS DILLHHLSKE
121PFLRGQGIDC ETLPEISNAD SFEEEAIIKS IISCYNKNSW PKEQTPELTD QLNPKRDGEN
181SNKPGSATTT EENTSDLEGP VAAGDSSHQE NVNVLTKTKG PGDKQKSYQG QSPQKQQTEK
241ANSGNTFKYG QGQVHYQLPD FSKIAPKVKI PKNKIINKPL AIAKQASFSS KSRDKPTLVQ
301DSLETTPESN CVEKQHQEQK GKITEPSQQI QMEPIVHIHQ ELLTGIESEA SLSKLSPTSQ
361KGTSSSSSYI FQKISQGKQM CQKLKEQTDQ LKTKVQEFSK RIKQDSPYHL QDKKLVLEKL
421QGHLELLEQN FLATKDKHLT LQQQVHKHES TIVGDFDPER KVEGEIFKLE MLLEDVKEKM
481DESKYTSAPS LPVSSPVTLD DLASTFSSLS NEIPKEHPGH PSGPRGSGGS EVTGTPQGGP
541QEAPNEELCE LAPQTYLNGH YGDAAAQNKP DQVAMRLSSN SGEDPNGTPR RQDCAEMTAP
601SPSCAFCRRL LEWKQNVEKK GHGRINCGRF SIVLHEKAPH SDSTPNSDTG HSFCSDSGTE
661MQSNKCQDCG TKIPTSRRAC RKEPTKEFHY RYNTPGQNYS NHSKRGAFVQ PHSLDESKNS
721SPSFLKPKRI CSQRVNSKSF KGEHEPTPGK KKLQAFMTYS SDPATPSPHF YSCRISGSKS
781LCDFDSTEEI KSEILNSALD HALRTATILK ETTDQMIKTI AEDLAKAQRW RNRLKY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

836 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.