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AKAP8L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AKAP8L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G114000-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AKAP8L (NM_014371), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A-kinase anchor protein 8-like | AKAP8-like protein | AKAP8L | HA95 | HAP95 | Helicase A-binding protein 95 | Neighbor of AKAP95 | Homologous to AKAP95 protein | NAKAP | NAKAP95
Origin Species
Human
NCBI GenBank
NM_014371
SwissProt
Q9ULX6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSYTGFVQGS ETTLQSTYSD TSAQPTCDYG YGTWNSGTNR GYEGYGYGYG YGQDNTTNYG
61YGMATSHSWE MPSSDTNANT SASGSASADS VLSRINQRLD MVPHLETDMM QGGVYGSGGE
121RYDSYESCDS RAVLSERDLY RSGYDYSELD PEMEMAYEGQ YDAYRDQFRM RGNDTFGPRA
181QGWARDARSG RPMASGYGRM WEDPMGARGQ CMSGASRLPS LFSQNIIPEY GMFQGMRGGG
241AFPGGSRFGF GFGNGMKQMR RTWKTWTTAD FRTKKKKRKQ GGSPDEPDSK ATRTDCSDNS
301DSDNDEGTEG EATEGLEGTE AVEKGSRVDG EDEEGKEDGR EEGKEDPEKG ALTTQDENGQ
361TKRKLQAGKK SQDKQKKRQR DRMVERIQFV CSLCKYRTFY EDEMASHLDS KFHKEHFKYV
421GTKLPKQTAD FLQEYVTNKT KKTEELRKTV EDLDGLIQQI YRDQDLTQEI AMEHFVKKVE
481AAHCAACDLF IPMQFGIIQK HLKTMDHNRN RRLMMEQSKK SSLMVARSIL NNKLISKKLE
541RYLKGENPFT DSPEEEKEQE EAEGGALDEG AQGEAAGISE GAEGVPAQPP VPPEPAPGAV
601SPPPPPPPEE EEEGAVPLLG GALQRQIRGI PGLDVEDDEE GGGGAPTRTR PLEQKLISEE
661DLAANDILDY KDDDDKV

677 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.