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AKAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AKAP2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AKAP2 antibodies →
$725.00each
Catalog Number: LS-G122543-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AKAP2 (NM_001004065), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AKAP-KL | AKAP-2 | AKAPKL | KIAA0920 | PALM2-AKAP2 | PALM2-AKAP2 protein | PALM2-AKAP2 readthrough | PRKA2
Origin Species
Human
NCBI GenBank
NM_001004065
SwissProt
Q9Y2D5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRWPQPGAAA RLPPESPGPP ESPGPPEREA AAARRWTGAE PQDCAPGSGR PEKPPQLSED
61DIWLKSEGDN YSATLLEPAA SSLSPDHKNM EIEVSVAECK SVPGITSTPH PMDHPSAFYS
121PPHNGLLTDH HESLDNDVAR EIRYLDEVLE ANCCDSAVDG TYNGTSSPEP GAVVLVGGLS
181PPVHEATQPE PTERTASRQA PPHIELSNSS PDPMAEAERT NGHSPSQPRD ALGDSLQVPV
241SPSSTTSSRC SSRDGEFTLT TLKKEAKFEL RAFHEDKKPS KLFEDDEHEK EQYCIRKVRP
301SEEMLELEKE RRELIRSQAV KKNPGIAAKW WNPPQEKTIE EQLDEEHLES HKKYKERKER
361RAQQEQLLLQ KQLQQQQQQP PSQLCTAPAS SHERASMIDK AKEDIVTEQI DFSAARKQFQ
421LMENSRQAVA KGQSTPRLFS IKPFYRPLGS VNSDKPLTNP RPPSVGGPPE DSGASAAKGQ
481KSPGALETPS AAGSQGNTAS QGKEGPYSEP SKRGPLSKLW AEDGEFTSAR AVLTVVKDDD
541HGILDQFSRS VNVSLTQEEL DSGLDELSVR SQDTTVLETL SNDFSMDNIS DSGASNETTN
601ALQENSLADF SLPQTPQTDN PSEGRGEGVS KSFSDHGFYS PSSTLGDSPL VDDPLEYQAG
661LLVQNAIQQA IAEQVDKAVS KTSRDGAEQQ GPEATVEEAE AAAFGSEKPQ SMFEPPQVSS
721PVQEKRDVLP KILPAEDRAL RERGPPQPLP AVQPSGPINM EETRPEGSYF SKYSEAAELR
781STASLLATQE SDVMVGPFKL RSRKQRTLSM IEEEIRAAQE REEELKRQRQ VLQSTQSPRT
841KNAPSLPSRT CYKTAPGKIE KVKPPPSPTT EGPSLQPDLA PEEAAGTQRP KNLMQTLMED
901YETHKSKRRE RMDDSSVLEA TRVNRRKSAL ALRWEAGIYA NQEEEDNE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

948 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.