Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AKAP17A / 721P Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116190-100
Catalog Number: LS-G116190
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFRS17A(AKAP17A) (NM_005088), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A-kinase anchor protein 17A | 721P | AKAP-17A | AKAP17A | B-lymphocyte antigen | B-lymphocyte surface antigen | CCDC133 | CXYorf3 | DXYS155E | PRKA17A | Pseudoautosomal gene XE7 | XE7Y | XE7 | Protein XE7 | SFRS17A
- Origin Species
- Human
- NCBI GenBank
- NM_005088
- SwissProt
- Q02040
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 80.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAATIVHDT SEAVELCPAY GLYLKPITKM TISVALPQLK QPGKSISNWE VMERLKGMVQ61NHQFSTLRIS KSTMDFIRFE GEVENKSLVK SFLACLDGKT IKLSGFSDIL KVRAAEFKID121FPTRHDWDSF FRDAKDMNET LPGERPDTIH LEGLPCKWFA LKESGSEKPS EDVLVKVFEK181FGEIRNVDIP MLDPYREEMT GRNFHTFSFG GHLNFEAYVQ YREYMGFIQA MSALRGMKLM241YKGEDGKAVA CNIKVSFDST KHLSDASIKK RQLERQKLQE LEQQREEQKR REKEAEERQR301AEERKQKELE ELERERKREE KLRKREQKQR DRELRRNQKK LEKLQAEEQK QLQEKIKLEE361RKLLLAQRNL QSIRLIAELL SRAKAVKLRE QEQKEEKLRL QQQEERRRLQ EAELRRVEEE421KERALGLQRK ERELRERLLS ILLSKKPDDS HTHDELGVAH ADLLQPVLDI LQTVSSGCVS481ATTLHPLGGQ PPAGAPKESP AHPEADGAPK SVNGSVAEEA PCKEVQSSCR VVPEDGSPEK541RCPGGVLSCI PDNNQQPKGI PACEQNVSRK DTRSEQDKCN REPSKGRGRA TGDGLADRHK601RERSRARRAS SREDGRPRKE RRPHKKHAYK DDSPRRRSTS PDHTRSRRSH SKDRHRRERS661RERRGSASRK HSRHRRRSER SRSRSPSRHR STWNRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
695 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.