Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AK9 / AKD1 / AKD2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106800-100
Catalog Number: LS-G106800
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C6ORF199(AK9) (NM_145025), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AKD2 | C6orf199 | C6orf224 | DJ70A9.1 | AKD1 | RP1-70A9.1
- Origin Species
- Human
- SwissProt
- Q5TCS8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSQEKTEEY PFADIFDEDE TERNFLLSKP VCFVVFGKPG VGKTTLARYI TQAWKCIRVE61ALPILEEQIA AETESGVMLQ SMLISGQSIP DELVIKLMLE KLNSPEVCHF GYIITEIPSL121SQDAMTTLQQ IELIKNLNLK PDVIINIKCP DYDLCQRISG QRQHNNTGYI YSRDQWDPEV181IENHRKKKKE AQKDGKGEEE EEEEEQEEEE AFIAEMQMVA EILHHLVQRP EDYLENVENI241VKLYKETILQ TLEEVMAEHN PQYLIELNGN KPAEELFMIV MDRLKYLNLK RAAILTKLQG301AEEEINDTME NDELFRTLAS YKLIAPRYRW QRSKWGRTCP VNLKDGNIYS GLPDYSVSFL361GKIYCLSSEE ALKPFLLNPR PYLLPPMPGP PCKVFILGPQ YSGKTTLCNM LAENYKGKVT421NTRTRPLEQKLISEEDLAANDILDYKDDDDKV
421 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.