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AK8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AK8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106168-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf98(AK8) (NM_152572), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AK8 | Adenylate kinase 8 | C9orf98 | RP11-143F18.1
Origin Species
Human
NCBI GenBank
NM_152572
SwissProt
Q96MA6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDATIAPHRI PPEMPQYGEE NHIFELMQNM LEQLLIHQPE DPIPFMIQHL HRDNDNVPRI
61VILGPPASGK TTIAMWLCKH LNSSLLTLEN LILNEFSYTA TEARRLYLQR KTVPSALLVQ
121LIQERLAEED CIKQGWILDG IPETREQALR IQTLGITPRH VIVLSAPDTV LIERNLGKRI
181DPQTGEIYHT TFDWPPESEI QNRLMVPEDI SELETAQKLL EYHRNIVRVI PSYPKILKVI
241SADQPCVDVF YQALTYVQSN HRTNAPFTPR VLLLGPVGSG KSLQAALLAQ KYRLVNVCCG
301QLLKEAVADR TTFGELIQPF FEKEMAVPDS LLMKVLSQRL DQQDCIQKGW VLHGVPRDLD
361QAHLLNRLGY NPNRVFFLNV PFDSIMERLT LRRIDPVTGE RYHLMYKPPP TMEIQARLLQ
421NPKDAEEQVK LKMDLFYRNS ADLEQLYGSA ITLNGDQDPY TVFEYIESGI INPLPKKIP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.