Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AIM / CD5L Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100677-100
Catalog Number: LS-G100677
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AIM(CD5L) (NM_005894), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIM | API6 | Apoptosis inhibitor 6 | CD5 antigen-like | CD5L | CT-2 | IgM-associated peptide | PRO229 | SP-ALPHA | CD5 molecule-like | Spalpha
- Origin Species
- Hu
- NCBI GenBank
- NM_005894
- SwissProt
- O43866
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 37.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALLFSLILA ICTRPGFLAS PSGVRLVGGL HRCEGRVEVE QKGQWGTVCD DGWDIKDVAV61LCRELGCGAA SGTPSGILYE PPAEKEQKVL IQSVSCTGTE DTLAQCEQEE VYDCSHDEDA121GASCENPESS FSPVPEGVRL ADGPGHCKGR VEVKHQNQWY TVCQTGWSLR AAKVVCRQLG181CGRAVLTQKR CNKHAYGRKP IWLSQMSCSG REATLQDCPS GPWGKNTCNH DEDTWVECED241PFDLRLVGGD NLCSGRLEVL HKGVWGSVCD DNWGEKEDQV VCKQLGCGKS LSPSFRDRKC301YGPGVGRIWL DNVRCSGEEQ SLEQCQHRFW GFHDCTHQED VAVICSGSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
347 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.