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AIFM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AIFM3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128271-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AIFM3 (NM_001146288), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIFL | AIFM3 | Apoptosis-inducing factor 3 | Apoptosis-inducing factor like
Origin Species
Human
SwissProt
Q96NN9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGGCFSKPKP GAALPTVELK IEVVLPEKER GKEELSASGK GSPRAYQGNG TARHFHTEER
61LSTPHPYPSP QDCVEAAVCH VKDLENGQMR EVELGWGKVL LVKDNGEFHA LGHKCPHYGA
121PLVKGVLSRG RVRCPWHGAC FNISTGDLED FPGLDSLHKF QVKIEKEKVY VRASKQALQL
181QRRTKVMAKC ISPSAGYSSS TNVLIVGAGA AGLVCAETLR QEGFSDRIVL CTLDRHLPYD
241RPKLSKSLDT QPEQLALRPK EFFRAYGIEV LTEAQVVTVD VRTKKVVFKD GFKLEYSKLL
301LAPGSSPKTL SCKGKEVENV FTIRTPEDAN RVVRLARGRN VVVVGAGFLG MEVAAYLTEK
361AHSVSVVELE ETPFRRFLGE RVGRALMKMF ENNRVKFYMQ TEVSELRGQE GKLKEVVLKS
421SKVVRADVCV VGIGAVPATG FLRQSGIGLD SRGFIPVNKM MQTNVPGVFA AGDAVTFPLA
481WRNNRKVNIP HWQMAHAQGR VAAQNMLAQE AEMSTVPYLW TAMFGKSLRY AGYGEGFDDV
541IIQGDLEELK FVAFYTKGDE VIAVASMNYD PIVSKVAEVL ASGRAIRKRE VETGDMSWLT
601GKGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

604 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.