Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AIFM1 / AIF / PDCD8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106275-100
Catalog Number: LS-G106275
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AIF(AIFM1) (NM_145812), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AIF | AIFM1 | COXPD6 | PDCD8 | Programmed cell death 8
- Origin Species
- Human
- NCBI GenBank
- NM_004208
- SwissProt
- O95831
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFRCGGLAAG ALKQKLVPLV RTVCVRSPRQ RNRLPVVQSH HLGSPSRSLA STGASGKDGS61NLVYFLIVGA TVTGAGVYYA YKTMKEDEKR YNERISGLGL TPEQKQKKAA LSASEGEEVP121QDKAPSHVPF LLIGGGTAAF AAARSIRARD PGARVLIVSE DPELPYMRPP LSKELWFSDD181PNVTKTLRFK QWNGKERSIY FQPPSFYVSA QDLPHIENGG VAVLTGKKVV QLDVRDNMVK241LNDGSQITYE KCLIATGGTP RSLSAIDRAG AEVKSRTTLF RKIGDFRSLE KISREVKSIT301IIGGGFLGSE LACALGRKAR ALGTEVIQLF PEKGNMGKIL PEYLSNWTME KVRREGVKVM361PNAIVQSVGV SSGKLLIKLK DGRKVETDHI VAAVGLEPNV ELAKTGGLEI DSDFGGFRVN421AELQARSNIW VAGDAACFYD IKLGRRRVEH HDHAVVSGRL AGENMTGAAK PYWHQSMFWS481DLGPDVGYEA IGLVDSSLPT VGVFAKATAQ DNPKSATEQS GTGIRSESET ESEASEITIP541PSTPAVPQAP VQGEDYGKGV IFYLRDKVVV GIVLWNIFNR MPIARKIIKD GEQHEDLNEV601AKLFNIHEDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
609 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.