Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AGTR2 / AT2 Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G99112-100
Catalog Number: LS-G99112
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Angiotensin II Type 2 Receptor(AGTR2) (NM_000686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Angiotensin ii receptor type 2 | Angiotensin II type-2 receptor | AT2 receptor | AT2 | At2r | AGTR2 | MRX88 | Angiotensin ii at2 | Angiotensin receptor 2 | Type-2 angiotensin II receptor | ATGR2
- NCBI GenBank
- NM_000686
- SwissProt
- P50052
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 41 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKGNSTLATT SKNITSGLHF GLVNISGNNE STLNCSQKPS DKHLDAIPIL YYIIFVIGFL61VNIVVVTLFC CQKGPKKVSS IYIFNLAVAD LLLLATLPLW ATYYSYRYDW LFGPVMCKVF121GSFLTLNMFA SIFFITCMSV DRYQSVIYPF LSQRRNPWQA SYIVPLVWRM ACLSSLPTFY181FRDVRTIEYL GVNACIMAFP PEKYAQWSAG IALMKNILGF IIPLIFIATC YFGIRKHLLK241TNSYGKNRIT RDQVLKMAAA VVLAFIICWL PFHVLTFLDA LAWMGVINSC EVIAVIDLAL301PFAILLGFTN SCVNPFLYCF VGNRFQQKLR SVFRVPITWL QGKRESMSCR KSSSLREMET361FVSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
363 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.