Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AGPAT9 / MAG1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102078-100
Catalog Number: LS-G102078
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAG1(GPAT3) (NM_032717), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 1-AGPAT 9 | 1-AGP acyltransferase 9 | AGPAT9 | AGPAT8 | GPAT-3 | GPAT3 | HMFN0839 | HGPAT3 | LPAAT-theta | MAG-1 | MAG1
- Origin Species
- Human
- NCBI GenBank
- NM_032717
- SwissProt
- Q53EU6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEGAELAGKI LSTWLTLVLG FILLPSVFGV SLGISEIYMK ILVKTLEWAT IRIEKGTPKE61SILKNSASVG IIQRDESPME KGLSGLRGRD FELSDVFYFS KKGLEAIVED EVTQRFSSEE121LVSWNLLTRT NVNFQYISLR LTMVWVLGVI VRYCVLLPLR VTLAFIGISL LVIGTTLVGQ181LPDSSLKNWL SELVHLTCCR ICVRALSGTI HYHNKQYRPQ KGGICVANHT SPIDVLILTT241DGCYAMVGQV HGGLMGIIQR AMVKACPHVW FERSEMKDRH LVTKRLKEHI ADKKKLPILI301FPEGTCINNT SVMMFKKGSF EIGGTIHPVA IKYNPQFGDA FWNSSKYNMV SYLLRMMTSW361AIVCDVWYMP PMTREEGEDA VQFANRVKSA IAIQGGLTEL PWDGGLKRAK VKDIFKEEQQ421KNYSKMIVGN GSLSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
434 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.