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AGFG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AGFG1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G125424-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGFG1 (NM_001135187), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AGFG1 | HRIP | Rev interacting protein | HIV-1 Rev binding protein | HIV-1 Rev-binding protein | HRB | Rev-interacting protein | RIP | Nucleoporin-like protein RIP | RAB | ArfGAP with FG repeats 1
Origin Species
Human
NCBI GenBank
NM_004504
SwissProt
P52594

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASAKRKQE EKHLKMLRDM TGLPHNRKCF DCDQRGPTYV NMTVGSFVCT SCSGSLRGLN
61PPHRVKSISM TTFTQQEIEF LQKHGNEVCK QIWLGLFDDR SSAIPDFRDP QKVKEFLQEK
121YEKKRWYVPP EQAKVVASVH ASISGSSASS TSSTPEVKPL KSLLGDSAPT LHLNKGTPSQ
181SPVVGRSQGQ QQEKKQFDLL SDLGSDIFAA PAPQSTATAN FANFAHFNSH AAQNSANADF
241ANFDAFGQSS GSSNFGGFPT ASHSPFQPQT TAFRMLSSSC SFGEFTSAFP LQATHSGSAA
301SVNANFAHFD NFPKSSSADF GTFNTSQSHQ TASAVSKVST NKAGLQTADK YAALANLDNI
361FSAGQGGDQG SGFGTTGKAP VGSVVSVPSQ SSASSDKYAA LAELDSVFSS AATSSNAYTS
421TSNASSNVFG TVPVVASAQT QPASSSVPAP FGATPSTNPF VAAAGPSVAS STNPFQTNAR
481GATAATFGTA SMSMPTGFGT PAPYSLPTSF SGSFQQPAFP AQAAFPQQTA FSQQPNGFAA
541FGQTKPVVTP FGQVAAAGVS SNPFMTGAPT GQFPTGSSST NPFL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

584 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.