Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AGBL2 / CCP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109738-100
Catalog Number: LS-G109738
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGBL2 (NM_024783), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGBL2 | ATP/GTP-binding protein-like 2 | ATP/GTP binding protein-like 2 | CCP2 | Cytosolic carboxypeptidase 2
- Origin Species
- Human
- NCBI GenBank
- NM_024783
- SwissProt
- Q5U5Z8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 104 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MFPALETHLK QTIPDPYEDF MYRHLQYYGY FKAQRGSLPN SATHQHVRKN NPQCLLNGSL61GEKDDLIPDT LQKEKLLWPI SLSSAVHRQI EAINRDFHSC LGWMQWRGLS SLQPPPPRFK121DSPASAFRVA GITDSHMLSL PHLRSRQLLY DELDEVNPRL REPQELFSIL STKRPLQAPR181WPIECEVIKE NIHHIEWAPP QPEYFYQPKG NEKVPEIVGE KKGTVVYQLD SVPIEGSYFT241SSRVGGKRGI VKELAVTLQG PEDNTLLFES RFESGNLQKA VRVDTYEYEL TLRTDLYTNK301HTQWFYFRVQ NTRKDATYRF TIVNLLKPKS LYTVGMKPLL YSQLDANTRN IGWRREGNEI361KYYKNNTDDG QQPFYCLTWT IQFPYDQDTC FFAHFYPYTY TDLQCYLLSV ANNPIQSQFC421KLQTLCRSLA GNTVYLLTIT NPSQTPQEAA AKKAVVLSAR VHPGESNGSW VMKGFLDFIL481SNSPDAQLLR DIFVFKVLPM LNPDGVIVGN YRCSLAGRDL NRHYKTILKE SFPCIWYTRN541MIKRLLEERE VLLYCDFHGH SRKNNIFLYG CNNNNRKYWL HERVFPLMLC KNAPDKFSFH601SCNFKVQKCK EGTGRVVMWR MGILNSYTME STFGGSTLGN KRDTHFTIED LKSLGYHVCD661TLLDFCDPDQ IKFTQCLAEL KELLRQEIHK KFHELGQDVD LEGSWSDISL SDIESSTSGS721DSSLSDGLPV HLANIADELT QKKKMFKKKK KKSLQTRKQR NEQYQKKNLM QKLKLTEDTS781EKAGFASTLQ KQPTFFKNSE NSSFLPMKNE NPRLNETNLN RRDKDTPLDP SMATLILPKN841KGRMQNKKPG FTVSCSPKRT INSSQEPAPG MKPNWPRSRY PATKRGCAAM AAYPSLHIYT901YPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
902 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.