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AGAP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AGAP5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G126295-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGAP5 (NM_001144000), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001144000

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNILTCCVH PSVSLEFDQQ QGSVCPSESE IYEAGAGDRM AGAPMAAAVQ PAEVTVEVGE
61DLHMHHIRDQ EMPEALEFSL SANPEASTIF QRNSQTDALE FNPSANPEAS TIFQRNSQTD
121VVEIRRSNCT NHVSTERFSQ QYSSCSTIFL DDSTASQHYL TMTIISVTLE IPHHITQRDA
181DRSLSIPDEQ LHSFAVSTVH ITKNRNGGGS LNNYSSSIPS TPSTSQEDPQ FSVPPTANTP
241TPVCKRSMRW SNLFTSEKGS HPDKERKAPE NHADTIGSGR AIPIKQGMLL KRSGKWLKTW
301KKKYVTLCSN GVLTYYSSLG DYMKNIHKKE IDLRTSTIKV PGKWPSLATS ACAPISSSKS
361NGLSKDMDTG LGDSICFSPS ISSTTSPKLN PPPSPHANKK KHLKKKSTNN FMIVSATGQT
421WHFEATTYEE RDAWVQAIQS QILASLQSCE SSKSKSQLTS QSEAMALQSI QNMRGNAHCV
481DYETQNPKWA SLNLGVLMCI ECSGIHRSLG TRLSRVRSLE LDDWPVELRK VMSSIGNDLA
541NSIWEGSSQG QTKPSVKSTR EEKERWIRSK YEEKLFLAPL PCTELSLGQH LLRATADEDL
601QTAILLLAHG SREEVNETCG EGDGCTALHL ACRKGNVVLA QLLIWYGVDV MARDAHGNTA
661LTYARQASSQ ECINVLLQYG CPDECV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

686 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.