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AGAP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AGAP4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107494-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AGAP8(AGAP4) (NM_133446), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AGAP-4 | AGAP4 | AGAP-8 | AGAP8 | CTGLF5 | Em:AC012044.1 | MRIP2 | ARF GTPase-activating protein | CTGLF1
Origin Species
Human
NCBI GenBank
NM_133446

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNILTCRVH PSVSLEFDQQ QGSVCPSESE IYEAGAGDRM AGAPMAAAVQ PAEVTVEVGE
61DLHMHHVRDR EMPEALEFNP SANPEASTIF QRNSQTDVVE IRRSNCTNHV STVRFSQQYS
121LCSTIFLDDS TAIQHYLTMT IISVTLEIPH HITQRDADRS LSIPDEQLHS FAVSTVHIMK
181KRNGGGSLNN YSSSIPSTPS TSQEDPQFSV PPTANTPTPV CKRSMRWSNL FTSEKGSDPD
241KERKAPENHA DTIGSGRAIP IKQGMLLKRS GKWLKTWKKK YVTLCSNGVL TYYSSLGDYM
301KNIHKKEIDL QTSTIKVPGK WPSLATSACT PISTSKSNGL SKDMDTGLGD SICFSPSISS
361TTSPKLNPPP SPHANKKKHL KKKSTNNFMI VSATGQTWHF EATTYEERDA WVQAIQSQIL
421ASLQSCESSK SKSQLTSQSK AMALQSIQNM RGNAHCVDCE TQNPKWASLN LGVLMCIECS
481GIHRSLGTRL SRVRSLELDD WPVELRKVMS SIGNDLANSI WEGSSQGQTK PSEKSTREEK
541ERWIRSKYEE KLFLAPLPCT ELSLGQQLLR ATADEDLQTA ILLLAHGSRE EVNETCGEGD
601GCTALHLACR KGNVVLAQLL IWYGVDVMAR DAHGNTALTY ARQASSQECI NVLLQYGCPD
661KCV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

663 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.