Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AGAP3 / CENTG3 / MRIP-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109124-100
Catalog Number: LS-G109124
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CENTG3(AGAP3) (NM_031946), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AGAP-3 | AGAP3 | Centaurin | gamma 3 | Centaurin-gamma-3 | CRAG | CRAM-associated GTPase | MRIP-1 | CENTG3 | Cnt-g3 | MR1-interacting protein
- Origin Species
- Human
- NCBI GenBank
- NM_031946
- SwissProt
- Q96P47
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 97.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MNFQAGGGQS PQQQQSLAAP GGGGAAAQQL VCGGQFGGAG PGAGGGGGPS QQLAGGPPQQ61FALSNSAAIR AEIQRFESVH PNIYAIYDLI ERIEDLALQN QIREHVISIE DSFVNSQEWT121LSRSVPELKV GIVGNLSSGK SALVHRYLTG TYVQEESPEG GRFKKEIVVD GQSYLLLIRD181EGGPPELQFA AWVDAVVFVF SLEDEISFQT VYNYFLRLCS FRNASEVPMV LVGTQDAISA241ANPRVIDDSR ARKLSTDLKR CTYYETCATY GLNVERVFQD VAQKVVALRK KQQLAIGPCK301SLPNSPSHSA VSAASIPAVH INQATNGGGS AFSDYSSSVP STPSISQREL RIETIAASST361PTPIRKQSKR RSNIFTSRKG ADLDREKKAA ECKVDSIGSG RAIPIKQGIL LKRSGKSLNK421EWKKKYVTLC DNGLLTYHPS LHDYMQNIHG KEIDLLRTTV KVPGKRLPRA TPATAPGTSP481RANGLSVERS NTQLGGGTGA PHSASSASLH SERPLSSSAW AGPRPEGLHQ RSCSVSSADQ541WSEATTSLPP GMQHPASGPA EVLSSSPKLD PPPSPHSNRK KHRRKKSTGT PRPDGPSSAT601EEAEESFEFV VVSLTGQTWH FEASTAEERE LWVQSVQAQI LASLQGCRSA KDKTRLGNQN661AALAVQAVRT VRGNSFCIDC DAPNPDWASL NLGALMCIEC SGIHRHLGAH LSRVRSLDLD721DWPPELLAVM TAMGNALANS VWEGALGGYS KPGPDACREE KERWIRAKYE QKLFLAPLPS781SDVPLGQQLL RAVVEDDLRL LVMLLAHGSK EEVNETYGDG DGRTALHLSS AMANVVFTQL841LIWYGVDVRS RDARGLTPLA YARRAGSQEC ADILIQHGCP GEGCGLAPTP NREPANGTNP901SAELHRSPSL LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
911 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.