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AFTPH Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AFTPH Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all AFTPH antibodies →
$504.00each
Catalog Number: LS-G112329-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Aftiphilin(AFTPH) (NM_017657), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Aftiphilin | AFTPH | AFTH | Aftiphilin protein | Nbla10388
Origin Species
Human
NCBI GenBank
NM_017657
SwissProt
Q6ULP2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPDIIRMYS SSPPPLDNGA EDDDDDEFGE FGGFSEVSPS GVGFVDFDTP DYTRPKEEFV
61PSNHFMPIHE FSENVDSLTS FKSIKNGNDK DITAELSAPV KGQSDVLLST TSKEIISSEM
121LATSIDGMER PGNLNKVVEQ RQNVGTLESF SPGDFRTNMN VVHQNKQLES CNGEKPPCLE
181ILTNGFAVLE TVNPQGTDDL DNVADSKGRK PLSTHSTEYN LDSVPSPAEE FADFATFSKK
241ERIQLEEIEC AVLNDREALT IRENNKINRV NELNSVKEVA LGRSLDNKGD TDGEDQVCVS
301EISIVTNRGF SVEKQGLPTL QQDEFLQSGV QSKAWSLVDS ADNSEAIRRE QCKTEEKLDL
361LTSKCAHLCM DSVKTSDDEV GSPKEESRKF TNFQSPNIDP TEENDLDDSL SVKNGDSSND
421FVTCNDINED DFGDFGDFGS ASGSTPPFVT GTQDSMSDAT FEESSEHFPH FSEPGDDFGE
481FGDINAVSCQ EETILTKSDL KQTSDNLSEE CQLARKSSGT GTEPVAKLKN GQEGEIGHFD
541SVPNIQDDCN GFQDSDDFAD FSSAGPSQVV DWNAFEDEQK DSCSWAAFGD QQATESHHRK
601EAWQSHRTDE NIDTPGTPKT HSVPSATSKG AVASGHLQES ATSVQTALLN RLERIFEACF
661PSILVPDAEE EVTSLKHLLE TSTLPIKTRE ALPESGELLD VWTELQDIHD AHGLRYQWGG
721SHSNKKLLSS LGIDTRNILF TGNKKQPVIV PMYAAGLGML EPTKEPLKPL SAAEKIASIG
781QTATMSPDMN TCTSDQFQES LPPVQFDWSS SGLTNPLDGV DPELYELTTS KLEISTSSLK
841VTDAFARLMS TVEKTSTSTS RKPKREEHLS EEAIKVVAGL PDLTFMHAKV LMFPATLTPS
901TSSQEKADG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

909 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.