Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
AFAP1L2 / XB130 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G122753-100
Catalog Number: LS-G122753
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AFAP1L2 (NM_001001936), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AFAP1L2 | AFAP1-like protein 2 | CTB-1144G6.4 | KIAA1914 | XB130 | Em:AC005383.4
- Origin Species
- Human
- NCBI GenBank
- AK027470
- SwissProt
- Q8N4X5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 91.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MERYKALEQL LTELDDFLKI LDQENLSSTA LVKKSCLAEL LRLYTKSSSS DEEYIYMNKV61TINKQQNAES QGKAPEEQGL LPNGEPSQHS SAPQKSLPDL PPPKMIPERK QLAIPKTESP121EGYYEEAEPY DTSLNEDGEA VSSSYESYDE EDGSKGKSAP YQWPSPEAGI ELMRDARICA181FLWRKKWLGQ WAKQLCVIKD NRLLCYKSSK DHSPQLDVNL LGSSVIHKEK QVRKKEHKLK241ITPMNADVIV LGLQSKDQAE QWLRVIQEVS GLPSEGASEG NQYTPDAQRF NCQKPDIAEK301YLSASEYGSS VDGHPEVPET KDVKKKCSAG LKLSNLMNLG RKKSTSLEPV ERSLETSSYL361NVLVNSQWKS RWCSVRDNHL HFYQDRNRSK VAQQPLSLVG CEVVPDPSPD HLYSFRILHK421GEELAKLEAK SSEEMGHWLG LLLSESGSKT DPEEFTYDYV DADRVSCIVS AAKNSLLLMQ481RKFSEPNTYI DGLPSQDRQE ELYDDVDLSE LTAAVEPTEE ATPVADDPNE RESDRVYLDL541TPVKSFLHGP SSAQAQASSP TLSCLDNATE ALPADSGPGP TPDEPCIKCP ENLGEQQLES601LEPEDPSLRI TTVKIQTEQQ RISFPPSCPD AVVATPPGAS PPVKDRLRVT SAEIKLGKNR661TEAEVKRYTE EKERLEKKKE EIRGHLAQLR KEKRELKETL LKCTDKEVLA SLEQKLKEID721EECRGEESRR VDLELSIMEV KDNLKKAEAG PVTLGTTVDT THLENVSPRP KAVTPASAPD781CTPVNSATTL KNRPLSVVVT GKGTVLQKAK EWEKKGASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
818 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.