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AFAP1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

AFAP1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106297-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AFAP1L1 (NM_152406), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AFAP1L1 | AFAP1-like protein 1
Origin Species
Human
SwissProt
Q8TED9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRGQVLEQL LPELTGLLSL LDHEYLSDTT LEKKMAVASI LQSLQPLPAK EVSYLYVNTA
61DLHSGPSFVE SLFEEFDCDL SDLRDMPEDD GEPSKGASPE LAKSPRLRNA ADLPPPLPNK
121PPPEDYYEEA LPLGPGKSPE YISSHNGCSP SHSIVDGYYE DADSSYPATR VNGELKSSYN
181DSDAMSSSYE SYDEEEEEGK SPQPRHQWPS EEASMHLVRE CRICAFLLRK KRFGQWAKQL
241TVIREDQLLC YKSSKDRQPH LRLALDTCSI IYVPKDSRHK RHELRFTQGA TEVLVLALQS
301REQAEEWLKV IREVSKPVGG AEGVEVPRSP VLLCKLDLDK RLSQEKQTSD SDSVGVGDNC
361STLGRRETCD HGKGKKSSLA ELKGSMSRAA GRKITRIIGF SKKKTLADDL QTSSTEEEVP
421CCGYLNVLVN QGWKERWCRL KCNTLYFHKD HMDLRTHVNA IALQGCEVAP GFGPRHPFAF
481RILRNRQEVA ILEASCSEDM GRWLGLLLVE MGSRVTPEAL HYDYVDVETL TSIVSAGRNS
541FLYARSCQNQ WPEPRVYDDV PYEKMQDEEP ERPTGAQVKR HASSCSEKSH RVDPQVKVKR
601HASSANQYKY GKNRAEEDAR RYLVEKEKLE KEKETIRTEL IALRQEKREL KEAIRSSPGA
661KLKALEEAVA TLEAQCRAKE ERRIDLELKL VAVKERLQQS LAGGPALGLS VSSKPKSGET
721ANKPQNSVPE QPLPVNCVSE LRKRSPSIVA SNQGRVLQKA KEWEMKKT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

768 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.