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ADSSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADSSL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADSSL1 antibodies →
$725.00each
Catalog Number: LS-G103435-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADSSL1 (NM_199165), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AMPSase 1 | AdSS 1 | ADSS1 | ADSL1 | ADSSL1 | IMP--aspartate ligase 1
Origin Species
Human
SwissProt
Q8N142

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVGRSCGVAT QRQGGGQRPT NLALTLSSSP AHSTALPWLP PRSLQLLSGH SVPAQPTPHL
61PSACGGPTRV TLGEERAWRS HGSNAGGHTC LPRRTAGAGS LTPGGERGGN NAGHTVVVDG
121KEYDFHLLPS GIINTKAVSF IGNGVVIHLP GLFEEAEKNE KKGLKDWEKR LIISDRAHLV
181FDFHQAVDGL QEVQRQAQEG KNIGTTKKGI GPTYSSKAAR TGLRICDLLS DFDEFSSRFK
241NLAHQHQSMF PTLEIDIEGQ LKRLKGFAER IRPMVRDGVY FMYEALHGPP KKILVEGANA
301ALLDIDFGTY PFVTSSNCTV GGVCTGLGIP PQNIGDVYGV VKAYTTRVGI GAFPTEQINE
361IGGLLQTRGH EWGVTTGRKR RCGWLDLMIL RYAHMVNGFT ALALTKLDIL DVLGEVKVGV
421SYKLNGKRIP YFPANQEMLQ KVEVEYETLP GWKADTTGAR RWEDLPPQAQ NYIRFVENHV
481GVAVKWVGVG KSRESMIQLF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

500 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.