Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADRA1B / Adrenoceptor alpha 1B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123040-100
Catalog Number: LS-G123040
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human alpha 1b Adrenergic Receptor(ADRA1B) (NM_000679), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha 1b | Alpha-1B adrenergic receptor | Alpha-1b-adrenergic | Alpha-1B-adrenergic receptor | Alpha1b | ADRA1B | Adrenoceptor alpha 1B | Alpha-1B adrenoreceptor | Alpha1B-AR | Alpha1beta-AR | ALPHA1BAR | ADRA1 | Alpha-1B adrenoceptor | Alpha1b-adrenergic
- Origin Species
- Human
- NCBI GenBank
- NM_000679
- SwissProt
- P35368
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 56.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPDLDTGHN TSAPAHWGEL KNANFTGPNQ TSSNSTLPQL DITRAISVGL VLGAFILFAI61VGNILVILSV ACNRHLRTPT NYFIVNLAMA DLLLSFTVLP FSAALEVLGY WVLGRIFCDI121WAAVDVLCCT ASILSLCAIS IDRYIGVRYS LQYPTLVTRR KAILALLSVW VLSTVISIGP181LLGWKEPAPN DDKECGVTEE PFYALFSSLG SFYIPLAVIL VMYCRVYIVA KRTTKNLEAG241VMKEMSNSKE LTLRIHSKNF HEDTLSSTKA KGHNPRSSIA VKLFKFSREK KAAKTLGIVV301GMFILCWLPF FIALPLGSLF STLKPPDAVF KVVFWLGYFN SCLNPIIYPC SSKEFKRAFV361RILGCQCRGR GRRRRRRRRR LGGCAYTYRP WTRGGSLERS QSRKDSLDDS GSCLSGSQRT421LPSASPSPGY LGRGAPPPVE LCAFPEWKAP GALLSLPAPE PPGRRGRHDS GPLFTFKLLT481EPESPGTDGG ASNGGCEAAA DVANGQPGFK SNMPLAPGQFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
520 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.