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ADPGK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADPGK Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109270-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADPGK (NM_031284), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADP-dependent glucokinase | ADPGK | ADP-GK | ATP-dependent glucokinase | RbBP-35
Origin Species
Human
NCBI GenBank
NM_031284
SwissProt
Q9BRR6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MALWRGSAYA GFLALAVGCV FLLEPELPGS ALRSLWSSLC LGPAPAPPGP VSPEGRLAAA
61WDALIVRPVR RWRRVAVGVN ACVDVVLSGV KLLQALGLSP GNGKDHSILH SRNDLEEAFI
121HFMGKGAAAE RFFSDKETFH DIAQVASEFP GAQHYVGGNA ALIGQKFAAN SDLKVLLCGP
181VGPKLHELLD DNVFVPPESL QEVDEFHLIL EYQAGEEWGQ LKAPHANRFI FSHDLSNGAM
241NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTSISDIPT GIPVHLELAS
301MTNRELMSSI VHQQVFPAVT SLGLNEQELL FLTQSASGPH SSLSSWNGVP DVGMVSDILF
361WILKEHGRSK SRASDLTRIH FHTLVYHILA TVDGHWANQL AAVAAGARVA GTQACATETI
421DTSRVSLRAP QEFMTSHSEA GSRIVLNPNK PVVEWHREGI SFHFTPVLVC KDPIRTVGLG
481DAISAEGLFY SEVHPHY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

497 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.