Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADORA1 / Adenosine A1 Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119403-100
Catalog Number: LS-G119403
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Adenosine A1 Receptor(ADORA1) (NM_001048230), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A1 adenosine receptor | A1ar | Adenosine A1 receptor | Adenosine receptor A1 | ADO-A1 receptor | ADORA1 | RDC7
- Origin Species
- Human
- NCBI GenBank
- NM_000674
- SwissProt
- P30542
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPPSISAFQA AYIGIEVLIA LVSVPGNVLV IWAVKVNQAL RDSTFCFIVP LAVADVAVGA61LVIPLAILIN IGPQTYFHTC LMVACPVLIL TQSSILALLA IAVDHYLRVK IPLRYKMVVT121PRRAAVAIAG CWILSFVVGL TPMFGWNNLS AVERAWAANG SMGEPVIKCE FEKVISMEYM181VYFNFFVWVL PPLLLMVLIY LEVFYLIRKQ LNKKVSASSG DPQKYYGKEL KIAKSLALIL241FLFALSWLPL HILNCITLFC QSCHKPSILT YIAIFLTHGN SAMNPIVYAF RIQKFRVTFL301KIWNDHFRCQ PAPPIDEDLP EERPDDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
326 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.