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ADNP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ADNP Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ADNP antibodies →
$504.00each
Catalog Number: LS-G113271-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ADNP (NM_015339), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADNP | ADNP homeobox 1 | ADNP1 | KIAA0784
Origin Species
Human
NCBI GenBank
NM_015339
SwissProt
Q9H2P0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFQLPVNNLG SLRKARKTVK KILSDIGLEY CKEHIEDFKQ FEPNDFYLKN TTWEDVGLWD
61PSLTKNQDYR TKPFCCSACP CSSKFFSAYK SHFRNVHSED FENRILLNCP YCTFNADKKT
121LETHIKIFHA PNASAPSSSL STFKDKNKND GLKPKQADSV EQAVYYCKKC TYRDPLYEIV
181RKHIYREHFQ HVAAPYIAKA GEKSLNGAVP LGSNAREESS IHCKRCLFMP KSYEALVQHV
241IEDHERIGYQ VTAMIGHTNV VVPRSKPLML IAPKPQDKKS MGLPPRIGSL ASGNVRSLPS
301QQMVNRLSIP KPNLNSTGVN MMSSVHLQQN NYGVKSVGQG YSVGQSMRLG LGGNAPVSIP
361QQSQSVKQLL PSGNGRSYGL GSEQRSQAPA RYSLQSANAS SLSSGQLKSP SLSQSQASRV
421LGQSSSKPAA AATGPPPGNT SSTQKWKICT ICNELFPENV YSVHFEKGHK AEKVPAVANY
481IMKIHNFTSK CLYCNRYLPT DTLLNHMLIH GLSCPYCRST FNDVEKMAAH MRMVHIDEEM
541GPKTDSTLSF DLTLQQGSHT NIHLLVTTYN LRDAPAESVA YHAQNNPPVP PKPQPKVQEK
601ADIPVKSSPQ AAVPYKKDVG KTLCPLCFSI LKGPISDALA HHLRERHQVI QTVHPVEKKL
661TYKCIHCLGV YTSNMTASTI TLHLVHCRGV GKTQNGQDKT NAPSRLNQSP SLAPVKRTYE
721QMEFPLLKKR KLDDDSDSPS FFEEKPEEPV VLALDPKGHE DDSYEARKSF LTKYFNKQPY
781PTRREIEKLA ASLWLWKSDI ASHFSNKRKK CVRDCEKYKP GVLLGFNMKE LNKVKHEMDF
841DAEWLFENHD EKDSRVNASK TADKKLNLGK EDDSSSDSFE NLEEESNESG SPFDPVFEVE
901PKISNDNPEE HVLKVIPEDA SESEEKLDQK EDGSKYETIH LTEEPTKLMH NASDSEVDQD
961DVVEWKDGAS PSESGPGSQQ VSDFEDNTCE MKPGTWSDES SQSEDARSSK PAAKKKATMQ
1021GDREQLKWKN SSYGKVEGFW SKDQSQWKNA SENDERLSNP QIEWQNSTID SEDGEQFDNM
1081TDGVAEPMHG SLAGVKLSSQ QA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1102 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.