Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRL4 / ELTD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101801-100
Catalog Number: LS-G101801
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ETL(ADGRL4) (NM_022159), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ELTD1 | ETL | ETL protein | KPG_003
- Origin Species
- Human
- NCBI GenBank
- BC025721
- SwissProt
- Q9HBW9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKRLPLLVVF STLLNCSYTQ NCTKTPCLPN AKCEIRNGIE ACYCNMGFSG NGVTICEDDN61ECGNLTQSCG ENANCTNTEG SYYCMCVPGF RSSSNQDRFI TNDGTVCIEN VNANCHLDNV121CIAANINKTL TKIRSIKEPV ALLQEVYRNS VTDLSPTDII TYIEILAESS SLLGYKNNTI181SAKDTLSNST LTEFVKTVNN FVQRDTFVVW DKLSVNHRRT HLTKLMHTVE QATLRISQSF241QKTTEFDTNS TDIALKVFFF DSYNMKHIHP HMNMDGDYIN IFPKRKAAYD SNGNVAVAFL301YYKSIGPLLS SSDNFLLKPQ NYDNSEEEER VISSVISVSM SSNPPTLYEL EKITFTLSHR361KVTDRYRSLC AFWNYSPDTM NGSWSSEGCE LTYSNETHTS CRCNHLTHFA ILMSSGPSIG421IKDYNILTRI TQLGIIISLI CLAICIFTFW FFSEIQSTRT TIHKNLCCSL FLAELVFLVG481INTNTNKLFC SIIAGLLHYF FLAAFAWMCI EGIHLYLIVV GVIYNKGFLH KNFYIFGYLS541PAVVVGFSAA LGYRYYGTTK VCWLSTENNF IWSFIGPACL IILVNLLAFG VIIYKVFRHT601AGLKPEVSCF ENIRSCARGA LALLFLLGTT WIFGVLHVVH ASVVTAYLFT VSNAFQGMFI661FLFLCVLSRK IQEEYYRLFK NVPCCFGCLRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
690 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.