Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRL2 / LPHN2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114528-100
Catalog Number: LS-G114528
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LPHN2(ADGRL2) (NM_012302), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cl2aa | CIRL2 | CL2 | Latrophilin 1 | Latrophilin-2 | Lectomedin-1 | Latrophilin 2 | Latrophilin homolog 2 (cow) | KIAA0786 | Beta-latrotoxin receptor | CIRL-2 | Latrophilin homolog 1 | Latrotoxin receptor 2 | Latrotoxin receptor homolog 2 | LEC1 | LPHH1 | LPHN2
- Origin Species
- Human
- NCBI GenBank
- NM_012302
- SwissProt
- O95490
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 157.18 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVSSGCRMRS LWFIIVISFL PNTEGFSRAA LPFGLVRREL SCEGYSIDLR CPGSDVIMIE61SANYGRTDDK ICDADPFQME NTDCYLPDAF KIMTQRCNNR TQCIVVTGSD VFPDPCPGTY121KYLEVQYECV PYIFVCPGTL KAIVDSPCIY EAEQKAGAWC KDPLQAADKI YFMPWTPYRT181DTLIEYASLE DFQNSRQTTT YKLPNRVDGT GFVVYDGAVF FNKERTRNIV KFDLRTRIKS241GEAIINYANY HDTSPYRWGG KTDIDLAVDE NGLWVIYATE QNNGMIVISQ LNPYTLRFEA301TWETVYDKRA ASNAFMICGV LYVVRSVYQD NESETGKNSI DYIYNTRLNR GEYVDVPFPN361QYQYIAAVDY NPRDNQLYVW NNNFILRYSL EFGPPDPAQV PTTAVTITSS AELFKTIIST421TSTTSQKGPM STTVAGSQEG SKGTKPPPAV STTKIPPITN IFPLPERFCE ALDSKGIKWP481QTQRGMMVER PCPKGTRGTA SYLCMISTGT WNPKGPDLSN CTSHWVNQLA QKIRSGENAA541SLANELAKHT KGPVFAGDVS SSVRLMEQLV DILDAQLQEL KPSEKDSAGR SYNKAIVDTV601DNLLRPEALE SWKHMNSSEQ AHTATMLLDT LEEGAFVLAD NLLEPTRVSM PTENIVLEVA661VLSTEGQIQD FKFPLGIKGA GSSIQLSANT VKQNSRNGLA KLVFIIYRSL GQFLSTENAT721IKLGADFIGR NSTIAVNSHV ISVSINKESS RVYLTDPVLF TLPHIDPDNY FNANCSFWNY781SERTMMGYWS TQGCKLVDTN KTRTTCACSH LTNFAILMAH REIAYKDGVH ELLLTVITWV841GIVISLVCLA ICIFTFCFFR GLQSDRNTIH KNLCINLFIA EFIFLIGIDK TKYAIACPIF901AGLLHFFFLA AFAWMCLEGV QLYLMLVEVF ESEYSRKKYY YVAGYLFPAT VVGVSAAIDY961KSYGTEKACW LHVDNYFIWS FIGPVTFIIL LNIIFLVITL CKMVKHSNTL KPDSSRLENI1021KSWVLGAFAL LCLLGLTWSF GLLFINEETI VMAYLFTIFN AFQGVFIFIF HCALQKKVRK1081EYGKCFRHSY CCGGLPTESP HSSVKASTTR TSARYSSGTQ SRIRRMWNDT VRKQSESSFI1141SGDINSTSTL NQGHSLNNAR DTSAMDTLPL NGNFNNSYSL HKGDYNDSVQ VVDCGLSLND1201TAFEKMIISE LVHNNLRGSS KTHNLELTLP VKPVIGGSSS EDDAIVADAS SLMHSDNPGL1261ELHHKELEAP LIPQRTHSLL YQPQKKVKSE GTDSYVSQLT AEAEDHLQSP NRDSLYTSMP1321NLRDSPYPES SPDMEEDLSP SRRSENEDIY YKSMPNLGAG HQLQMCYQIS RGNSDGYIIP1381INKEGCIPEG DVREGQMQLV TSLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1403 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.