Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ADGRG7 / GPR128 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G102085-100
Catalog Number: LS-G102085
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPR128(ADGRG7) (NM_032787), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G protein-coupled receptor 128 | GPR128
- Origin Species
- Human
- NCBI GenBank
- NM_032787
- SwissProt
- Q96K78
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 88.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASCRAWNLR VLVAVVCGLL TGIILGLGIW RIVIRIQRGK STSSSSTPTE FCRNGGTWEN61GRCICTEEWK GLRCTIANFC ENSTYMGFTF ARIPVGRYGP SLQTCGKDTP SAGNPMAVRL121CSLSLYGEIE LQKVTIGNCN ENLETLEKQV KDVTAPLNNI SSEVQILTSD ANKLTAENIT181SATRVVGQMF DTSRNASPEA KKVAIVTVSQ LLDASEDAFQ RVAATANDDA LTTLIEQMET241YSLSLGNQSV VEPNIAIQSA NFSSENAVGP SNVRFSVQKG ASSSLVSSST FIHTNVDGLN301PDAQTELQVL LNMTKNYTKT CGFVVYQNDK LFQSKTFTAK SDFSQKIISS KTDENEQDQS361ASVDMVFSPK YNQKEFQLYS YACVYWNLSA KDWDTYGCQK DKGTDGFLRC RCNHTTNFAV421LMTFKKDYQY PKSLDILSNV GCALSVTGLA LTVIFQIVTR KVRKTSVTWV LVNLCISMLI481FNLLFVFGIE NSNKNLQTSD GDINNIDFDN NDIPRTDTIN IPNPMCTAIA ALLHYFLLVT541FTWNALSAAQ PYYLLIRTMK PLPRHFILFI SLIGWGVPAI VVAITVGVIY SQNGNNPQWE601LDYRQEKICW LAIPEPNGVI KSPLLWSFIV PVTIILISNV VMFITISIKV LWKNNQNLTS661TKKVSSMKKI VSTLSVAVVF GITWILAYLM LVNDDSIRIV FSYIFCLFNA TQGLQIFILY721TVRTKVFQSE ASKVLMLLSS IGRRKSLPSV TRPRLRVKMY NFLRSLPTLH ERFRLLETSP781STEEITLSES DNAKESITRTRPLEQKLISEEDLAANDILDYKDDDDKV
797 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.